Firefly Scientific

Histology

Antigen Retrieval

Effective unmasking in order to detect the antigen that is otherwise concealed by the negative effects of formalin fixation has an undeniable and positive impact on the validation process.

The mechanism of these negative effects is not yet completely understood but it is presumed that heating tissue sections in Trilogy causes the break up of the formalin cross-links that are believed to interfere with antigen-antibody binding. The discovery of the benefits of effective unmasking has been a revolutionary step in paving the road to standardization and the establishment of reliable validation procedures.

Trilogy is the most innovative (US Patent 6,649,368 B1) method to standardize preparation of FFPE sections for immunostaining in a single step.

Six great reasons to standardise with TRILOGY™

• Deparafinizes, rehydrates & unmasks in one step
• Short, approx 35 min pre treatment time
• non toxic, non flamable & biodegradable
• Deparafinizing with heat is more effective than cold solvents
• Cost effective
• Standardise protocols giving reproducibility and efficiency